[multi_overview]

[multi_overview_desc]

[multi_compare]

  • [multi_benefit1]
  • [multi_benefit2]
  • [multi_benefit3]

[multi_sections]

  • [multi_exec_summary]
  • [multi_detailed]

[exec_summary_title]


[wavelength_analysis]

[analysis_n_of]

ELISA Analysis Report

Assay Type: ELISA
Analysis Date: 2026-02-10
Analyst: MOELLERKR

This report analyzes ELISA data using a four-parameter logistic dose–response curve to estimate concentrations of cortisol in unknown samples.

Method: Competitive enzyme-linked immunosorbent assay with %B/B0 normalization

Analysis Notes

No additional notes provided.

Standard Curve Configuration

Standard Concentrations
Standard Concentration (pg/mL)
1 4,000.0
2 1,600.0
3 640.0
4 256.0
5 102.4
6 41.0
7 16.4
8 6.6

Dose–Response Curve Analysis

✅ All standards show acceptable variability (<30% CV).

Model Parameters

Four-Parameter Logistic Model Coefficients
Parameter Estimate Std. Error P-value
b:(Intercept) Hill Slope 1.06 0.10 NA
c:(Intercept) Bottom 0.00 3.80 NA
d:(Intercept) Top 100.00 1.83 NA
e:(Intercept) IC50 378.33 39.53 NA

Model Fit Statistics:

  • = 0.995
  • RMSE = 2.488 %B/B0
  • Standards Used = 16 of 8

Standard Back-Calculation and Recovery

Standard Back-Calculation and Recovery
Nominal (pg/mL) n Back-Calc. (pg/mL) SD (pg/mL) Recovery (%)
4,000.0 2 4,784.14 294.1 119.60
1,600.0 2 1,594.29 159.4 99.64
640.0 2 597.26 49.0 93.32
256.0 2 266.32 13.2 104.03
102.4 2 115.46 31.4 112.75
41.0 2 30.72 17.6 74.92
16.4 2 15.14 16.2 92.30
6.6 2 NaN
## 
## **Overall Mean Recovery:** 99.5%
## ✅ Overall recovery is within acceptable range (80–120%).

Sample Concentration Results

## 
## **Output Files Created:**
## - `unknown_results.csv` - Individual well results
## - `unknown_results_summary.csv` - Replicate group statistics with confidence intervals
## 
## **⚠️ Quality Alert:**
## - Replicate groups with high variability (CV > 30%): AE, BD, BH 
## - Consider checking sample preparation or dilution consistency

Detailed Sample Results Summary

[detailed_caption]
Replicate Group Sample IDs Sample Type n Mean (pg/mL) SD (pg/mL) SE (pg/mL) 95% CI CV% Range
AA A4,A5 Sample 2 438.99 4.89 3.46 1.11 [395.0 – 482.9] ✅ |
AB C4,C5 Sample 2 30.22 6.92 4.89 22.89 [ 0.0 – 92.4] <LLOQ
AC E4,E5 Sample 2 1,150.11 17.66 12.49 1.54 [991.4 – 1308.8] ✅ |
AD G4,G5 Sample 2 890.87 103.89 73.46 11.66 [ 0.0 – 1824.3] ✅ |
AE B4,B5 Sample 2 43.37 17.26 12.20 39.79 [ 0.0 – 198.4] <LLOQ
AF D4,D5 Sample 2 32.83 5.11 3.61 15.56 [ 0.0 – 78.7] <LLOQ
AG F4,F5 Sample 2 942.78 30.48 21.55 3.23 [668.9 – 1216.6] ✅ |
AH H4,H5 Sample 2 810.20 24.51 17.33 3.03 [590.0 – 1030.4] ✅ |
BA A6,A7 Sample 2 952.81 53.05 37.51 5.57 [476.2 – 1429.4] ✅ |
BB C6,C7 Sample 2 1,724.14 193.09 136.54 11.20 [ 0.0 – 3459.0] >ULOQ
BC E6,E7 Sample 2 406.20 74.75 52.86 18.40 [ 0.0 – 1077.8] ✅ |
BD G6,G7 Sample 2 26.85 20.17 14.26 75.11 [ 0.0 – 208.1] <LLOQ
BE B6,B7 Sample 2 851.00 11.26 7.96 1.32 [749.8 – 952.2] ✅ |
BF D6,D7 Sample 2 770.88 58.74 41.54 7.62 [243.1 – 1298.6] ✅ |
BG F6,F7 Sample 2 87.30 7.25 5.13 8.31 [ 22.1 – 152.5] <LLOQ
BH H6,H7 Sample 2 32.92 12.55 8.87 38.12 [ 0.0 – 145.6] <LLOQ

Sample Variability Visualization

Dose-Response Curve with Unknown Samples


Report Generated: 2026-02-10
Contact:
Feedback: Online Form

Automated bioassay analysis using modular system v2.0


[wavelength_analysis]

[analysis_n_of]

ELISA Analysis Report

Assay Type: ELISA
Analysis Date: 2026-02-10
Analyst: MOELLERKR

This report analyzes ELISA data using a four-parameter logistic dose–response curve to estimate concentrations of cortisol in unknown samples.

Method: Competitive enzyme-linked immunosorbent assay with %B/B0 normalization

Analysis Notes

No additional notes provided.

Standard Curve Configuration

Standard Concentrations
Standard Concentration (pg/mL)
1 4,000.0
2 1,600.0
3 640.0
4 256.0
5 102.4
6 41.0
7 16.4
8 6.6

Dose–Response Curve Analysis

✅ All standards show acceptable variability (<30% CV).

Model Parameters

Four-Parameter Logistic Model Coefficients
Parameter Estimate Std. Error P-value
b:(Intercept) Hill Slope 1.05 0.09 NA
c:(Intercept) Bottom 0.00 3.68 NA
d:(Intercept) Top 100.00 1.80 NA
e:(Intercept) IC50 375.71 38.09 NA

Model Fit Statistics:

  • = 0.995
  • RMSE = 2.425 %B/B0
  • Standards Used = 16 of 8

Standard Back-Calculation and Recovery

Standard Back-Calculation and Recovery
Nominal (pg/mL) n Back-Calc. (pg/mL) SD (pg/mL) Recovery (%)
4,000.0 2 4,874.24 75.8 121.86
1,600.0 2 1,601.11 169.6 100.07
640.0 2 596.38 48.6 93.18
256.0 2 265.45 14.1 103.69
102.4 2 114.87 29.7 112.17
41.0 2 31.49 16.3 76.81
16.4 2 16.26 16.8 99.13
6.6 2 NaN
## 
## **Overall Mean Recovery:** 101.0%
## ✅ Overall recovery is within acceptable range (80–120%).

Sample Concentration Results

## 
## **Output Files Created:**
## - `unknown_results.csv` - Individual well results
## - `unknown_results_summary.csv` - Replicate group statistics with confidence intervals
## 
## **⚠️ Quality Alert:**
## - Replicate groups with high variability (CV > 30%): AE, BD, BH 
## - Consider checking sample preparation or dilution consistency

Detailed Sample Results Summary

[detailed_caption]
Replicate Group Sample IDs Sample Type n Mean (pg/mL) SD (pg/mL) SE (pg/mL) 95% CI CV% Range
AA A4,A5 Sample 2 439.25 4.86 3.44 1.11 [395.6 – 482.9] ✅ |
AB C4,C5 Sample 2 30.69 6.34 4.48 20.65 [ 0.0 – 87.6] <LLOQ
AC E4,E5 Sample 2 1,143.01 17.40 12.31 1.52 [986.7 – 1299.4] ✅ |
AD G4,G5 Sample 2 889.63 99.18 70.13 11.15 [ 0.0 – 1780.7] ✅ |
AE B4,B5 Sample 2 43.66 18.38 13.00 42.09 [ 0.0 – 208.8] <LLOQ
AF D4,D5 Sample 2 32.93 5.01 3.54 15.22 [ 0.0 – 78.0] <LLOQ
AG F4,F5 Sample 2 938.46 30.11 21.29 3.21 [667.9 – 1209.0] ✅ |
AH H4,H5 Sample 2 809.76 20.88 14.76 2.58 [622.2 – 997.3] ✅ |
BA A6,A7 Sample 2 948.04 43.65 30.87 4.60 [555.8 – 1340.3] ✅ |
BB C6,C7 Sample 2 1,731.31 204.69 144.74 11.82 [ 0.0 – 3570.4] >ULOQ
BC E6,E7 Sample 2 407.30 69.98 49.49 17.18 [ 0.0 – 1036.1] ✅ |
BD G6,G7 Sample 2 27.73 19.80 14.00 71.42 [ 0.0 – 205.6] <LLOQ
BE B6,B7 Sample 2 842.52 11.04 7.81 1.31 [743.3 – 941.7] ✅ |
BF D6,D7 Sample 2 766.34 61.15 43.24 7.98 [217.0 – 1315.7] ✅ |
BG F6,F7 Sample 2 88.35 5.52 3.90 6.25 [ 38.8 – 137.9] <LLOQ
BH H6,H7 Sample 2 33.64 10.50 7.43 31.22 [ 0.0 – 128.0] <LLOQ

Sample Variability Visualization

Dose-Response Curve with Unknown Samples


Report Generated: 2026-02-10
Contact:
Feedback: Online Form

Automated bioassay analysis using modular system v2.0


[wavelength_analysis]

[analysis_n_of]

ELISA Analysis Report

Assay Type: ELISA
Analysis Date: 2026-02-10
Analyst: MOELLERKR

This report analyzes ELISA data using a four-parameter logistic dose–response curve to estimate concentrations of cortisol in unknown samples.

Method: Competitive enzyme-linked immunosorbent assay with %B/B0 normalization

Analysis Notes

No additional notes provided.

Standard Curve Configuration

Standard Concentrations
Standard Concentration (pg/mL)
1 4,000.0
2 1,600.0
3 640.0
4 256.0
5 102.4
6 41.0
7 16.4
8 6.6

Dose–Response Curve Analysis

✅ All standards show acceptable variability (<30% CV).

Model Parameters

Four-Parameter Logistic Model Coefficients
Parameter Estimate Std. Error P-value
b:(Intercept) Hill Slope 1.05 0.09 NA
c:(Intercept) Bottom 0.00 3.71 NA
d:(Intercept) Top 100.00 1.79 NA
e:(Intercept) IC50 378.15 38.52 NA

Model Fit Statistics:

  • = 0.995
  • RMSE = 2.403 %B/B0
  • Standards Used = 16 of 8

Standard Back-Calculation and Recovery

Standard Back-Calculation and Recovery
Nominal (pg/mL) n Back-Calc. (pg/mL) SD (pg/mL) Recovery (%)
4,000.0 2 4,697.73 0.0 117.44
1,600.0 2 1,606.84 166.9 100.43
640.0 2 597.37 48.5 93.34
256.0 2 265.84 13.7 103.84
102.4 2 114.71 30.5 112.03
41.0 2 31.29 15.6 76.31
16.4 2 16.10 17.0 98.17
6.6 2 NaN
## 
## **Overall Mean Recovery:** 100.2%
## ✅ Overall recovery is within acceptable range (80–120%).

Sample Concentration Results

## 
## **Output Files Created:**
## - `unknown_results.csv` - Individual well results
## - `unknown_results_summary.csv` - Replicate group statistics with confidence intervals
## 
## **⚠️ Quality Alert:**
## - Replicate groups with high variability (CV > 30%): AE, BD, BH 
## - Consider checking sample preparation or dilution consistency

Detailed Sample Results Summary

[detailed_caption]
Replicate Group Sample IDs Sample Type n Mean (pg/mL) SD (pg/mL) SE (pg/mL) 95% CI CV% Range
AA A4,A5 Sample 2 439.82 5.10 3.61 1.159 [394.0 – 485.6] ✅ |
AB C4,C5 Sample 2 29.81 7.07 5.00 23.722 [ 0.0 – 93.3] <LLOQ
AC E4,E5 Sample 2 1,162.91 31.03 21.94 2.669 [884.1 – 1441.7] ✅ |
AD G4,G5 Sample 2 906.20 106.26 75.14 11.726 [ 0.0 – 1860.9] ✅ |
AE B4,B5 Sample 2 42.27 18.14 12.83 42.926 [ 0.0 – 205.3] <LLOQ
AF D4,D5 Sample 2 31.80 5.22 3.69 16.402 [ 0.0 – 78.7] <LLOQ
AG F4,F5 Sample 2 948.57 27.35 19.34 2.884 [702.8 – 1194.3] ✅ |
AH H4,H5 Sample 2 815.50 22.02 15.57 2.700 [617.7 – 1013.3] ✅ |
BA A6,A7 Sample 2 952.49 50.43 35.66 5.294 [499.4 – 1405.6] ✅ |
BB C6,C7 Sample 2 1,743.27 203.20 143.68 11.656 [ 0.0 – 3568.9] >ULOQ
BC E6,E7 Sample 2 409.70 72.21 51.06 17.625 [ 0.0 – 1058.5] ✅ |
BD G6,G7 Sample 2 26.70 19.20 13.57 71.906 [ 0.0 – 199.2] <LLOQ
BE B6,B7 Sample 2 847.30 7.75 5.48 0.914 [777.7 – 916.9] ✅ |
BF D6,D7 Sample 2 774.29 57.97 40.99 7.487 [253.4 – 1295.2] ✅ |
BG F6,F7 Sample 2 87.38 5.76 4.08 6.596 [ 35.6 – 139.2] <LLOQ
BH H6,H7 Sample 2 33.21 10.95 7.75 32.984 [ 0.0 – 131.6] <LLOQ

Sample Variability Visualization

Dose-Response Curve with Unknown Samples


Report Generated: 2026-02-10
Contact:
Feedback: Online Form

Automated bioassay analysis using modular system v2.0


[overall_conclusions]

[wavelength_performance]

[recommendations]

  • [rec_r2]
  • [rec_cv]
  • [rec_separation]

[report_generated] 2026-02-10 [contact] [feedback] [online_form]

[automated_multi]